Quantitive TLC Analysis

1. The "Instruction" Tab: Methodology and Usage Guide

This section is crucial for a full understanding of the application's capabilities. Here you will find:

• Comprehensive details on **all quantitative, qualitative, and optimization parameters** used in the chromatographic calculations.
• **Formulas and definitions** for calculating key parameters, along with diagrams illustrating how the application processes data.
• A detailed **step-by-step user guide** to all application functionalities.

2. The "Examples" Tab: Testing Data

The "Examples" tab contains TLC plate images utilized for testing the application throughout all stages of its development, from initial algorithms to its current state.

3. Other Projects: "V5" and "V6"

While **"V3"** is the primary version, we encourage you to explore our advanced projects:

• **V6 Algorithms:** A collection of independent algorithms that served to optimize the background reconstruction method. This advancement eliminates the need for image downscaling during background estimation.
• **V5 - Fully Automated Processing:** This version is designed for fully automatic data processing, from image upload to report generation (currently available in Polish only). Its architecture supports automatic modification by an AI in certain aspects, such as adjusting the number of images analyzed simultaneously for different light wavelengths, or modifying the visual layout. This may potentially simplify the workflow for non-technical users.

4. Important Disclaimers

PLEASE NOTE: This application was designed strictly for **educational purposes** and has **not been validated for quantitative analysis** of substances in a regulatory context. Only version **V3** has been tested in a real-world setting during classes with students at the University of Rzeszów.